Molecular biology of nucleases
- 282 Pages
- 2.43 MB
- 6875 Downloads
CRC Press , Boca Raton
|Statement||Nawin C. Mishra.|
|LC Classifications||QP609.N78 M57 1995|
|The Physical Object|
|Pagination||282 p. :|
|LC Control Number||94039830|
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Nucleases assist in the identification and characterization of genes responsible for several diseases and their possible alleviation by gene therapy. Molecular Biology of Nucleases introduces the properties and biological roles of nucleases.
Description Molecular biology of nucleases PDF
It is the one comprehensive source for Cited by: Book Description Nucleases occupy a central position in the biochemistry of DNA transactions and other metabolism of nucleic acids in all organisms. They have also proven useful in modern biological studies crucial for the development of recombinant DNA technology and reverse genetics.
An ideal text and reference work for professionals and students in biochemistry, genetics, and molecular biology, Nucleases promises to be the field’s standard-bearer for years to come.
Nucleases are a class of enzymes that catalyze the hydrolysis of nucleic acids (DNA, RNA) in all organisms, including by: Summary: Nucleases are an aid in the biochemistry of DNA transactions, and assist in the identification and characterization of genes responsible for certain diseases.
This book introduces the properties and other useful biological roles of nucleases. The chapters deal with mechanisms of RNA and DNA cleavage by chemical agents and natural enzymes, development of new nucleic acid-cleaving agents, applications of artificial nucleases, and design of oligonucleotide conjugates with RNA- and DNA-cleaving : Paperback.
The authors of the present volume summarize the state-of-the-art in the rapidly developing field of artificial nucleases. The chapters deal with mechanisms of RNA and DNA cleavage by chemical agents and natural enzymes, development of new nucleic acid-cleaving agents, applications of artificial nucleases, and design of oligonucleotide Molecular biology of nucleases book with RNA- and DNA-cleaving cturer: Springer.
N ucleases are also called nucleodepolymerase or polynucleotidase are enzymes able to cut off the phosphodiester bonds that join the adjacent nucleotides in a nucleic acid like RNA and DNA.
Nucleases are highly essential cellular machinery which is naturally present in biological components containing Nucleic acids. Summary: Provides detailed applications of nucleases in recombinant DNA technology, molecular cloning, biotechnology, pharmaceuticals, and commerce. This work covers the role of nucleases in biological systems, with focus on understanding their role in causing human diseases.
Nucleases are a class of enzymes that catalyze the hydrolysis of nucleic acids (DNA, RNA) in all organisms, including humans. In addition to their important biological role, nucleases have recently emerged as useful tools in laboratory studies, and have led to the development of such fields as recombinant DNA technology, molecular cloning, and genomics.
Nawin Mishra’s Nucleases, the first. "Professors, lecturers, and instructors will find the fifth edition of the book Molecular Biology of the Cell and its accompanying Problems Book Molecular biology of nucleases book be an excellent choice for guiding their students through the maze of the cell's molecular structures and biochemical countless colorful illustrations and a large number of photographs and tables, reading the text Reviews: DNA nucleases catalyze the cleavage of phosphodiester bonds between deoxyribose and the phosphate residue within the backbone of DNA strands.
Nucleases that cleave near the ends of DNA molecules are called exonucleases while enzymes that cleave within a DNA strand and do not require a free DNA end for cleavage are called endonucleases. The most basic function of the cell cycle is to duplicate accurately the vast amount of DNA in the chromosomes and then segregate the copies precisely into two genetically identical daughter cells.
These processes define the two major phases of the cell cycle. DNA duplication occurs during S phase (S for synthesis), which requires 10–12 hours and occupies about half of the cell-cycle time in.
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Large DNA Molecules Are Cut into Fragments by Restriction Nucleases. Unlike a protein, a gene does not exist as a discrete entity in cells, but rather as a small region of a much longer DNA gh the DNA molecules in a cell can be randomly broken into small pieces by mechanical force, a fragment containing a single gene in a mammalian genome would still be only one among a.
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Keywords: Enzymes, Molecular biology, Molecular cloning Introduction At a time when molecular cloning has become routine laboratory technique, we thought it was important to provide readers with some cues for understanding the function and specificities of the different enzymes used to generate and manipulate nucleic acids.
In molecular biology, the process whereby two complementary nucleic acid strands form a double helix. Forms the basis of a powerful technique for detecting specific nucleotide sequences.
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